Turbo Broth, Turbo Prime Broth and the new Turbo Prime-olate are proprietary media formulations used for the cultivation of recombinant strains of E. coli. Both Turbo Prime and Turbo Prime-olate are APF Certified. Turbo Prime-olate is made with Athena's proprietary plant protein hydrolysate: Atholate. The media improve the yield of recombinant proteins and typically produce cell yields 4 to 5 times higher than that of LB Broth in shake flask cultures depending on the strain. They have higher plasmid copy numbers than LB, which can enhance expression. Each medium uses glycerol for a carbon source and has a rich nutrient base of amino acids, vitamins, inorganic minerals, and trace minerals. The media are buffered at pH 7.2 with potassium phosphate to prevent a change in pH and provide a source of phosphate. These media are ideal as the base for fermentation cultures. Biomass yields will depend on the process employed and on the strain utilized.
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